Filters or monochromators for emission wavelength. Lyse cells Read on an HTRF compatible reader . Plate 25 μL of cells in. culture plate. At Cisbio we provide microplates that help you discover better. Revvity. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. FOR HTRF CERTIFIED READER. Customer Transformation Hub. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. In particular, these parameters should be entered as below. 96-well culture plateHowever, time-resolved fluorescence (TRF & TR-FRET) assays are used in conjunction with fluorophores that have a longer half-life. HTRF is compatible with a large range of available microplate readers. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. FOR HTRF CERTIFIED READER For. HTRF is a proprietary time-resolved fluorescence technology that overcomes Results summary for HTRF IP1 standard curve. For more information about HTRF. For more information about HTRF. Customer Transformation Hub. Read & analyse. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Push the screw down and turn it clockwise to lock the plate. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. PerkinElmer EnVision Multimode Plate Reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. For more information. FOR HTRF CERTIFIED READER For. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Lysates are then transferred to the assay plate for the detection of Total-eNOS by HTRF. FOR HTRF CERTIFIED READER. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Typical applications The HTRF cartridge provides optimal performance for key applications. a one- plate assay protocol. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. -Certified Reader. . LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. It comes equipped with our patented LVF Monochromators™, filters, and spectrometer. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. High Speed Monochromator offers a typical wavelength accuracy of <0. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Remove the plate sealer and read on an HTRF® compatible reader . 1) . It can also be further streamlined to a one-. best fluorescence polarization plate reader on the market. For more information. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. certified for use with HTRF. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. acceptor reagent. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate cells Stimulate cells 3. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. FOR HTRF CERTIFIED READER For more. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. FOR HTRF CERTIFIED READER. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. For more information about HTRF. For more information. Part Number# Product Description Number of Plates# 66PL96001 HTRF 96 well low volume plate 1 plate 66PL96005 HTRF 96 well low volume plate 5 platesThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The plate was covered and incubated for two hours at room temperature. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. For a list of HTRF-compatible readers and set-up recommendations, please visit . The optional HTRF-certified filter-based module may be. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Spark - Technical Notes. Make sure the setup for Tb Cryptate is used. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. It features a modular design that allows users to add or change detection cartridges according to their needs. • HTRF®-Certified Reader. Highly compatible plates for HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. The donor and acceptor can be used to label a variety of biomolecules, for. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PHERAstar FSX Powerful and most sensitive HTS plate reader . Incubate 2h at RT Transfer 16 µL supernatant. and Canaa -8-963-4567. This protocol gives the cells viability and confluence to be monitored. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. The Spark multimode reader from Tecan performs accurate and sensitive measurements of fluorescent cell-based assays. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Dual monochromators for assay flexibility With. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. 3. This protocol, HTS designed, allows miniaturization while. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It features a modular design that allows users to add or change detection cartridges according to their needs. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. The optical systems use two scanningHighly compatible plates for HTRF. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. FOR HTRF CERTIFIED READER For more information about HTRF. Smallest and most affordable multimode plate reader in the industry. The kit includes everything you need: reagents, buffers and a microplate. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. This protocol gives the cells viability and confluence to be monitored. Incubate overnight at RT. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. 41 mg/ml) was obtained from Thermo Fisher Scientific. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. It can also be further streamlined to a one-. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. FOR HTRF CERTIFIED READER. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. FOR HTRF CERTIFIED READER. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. • HTRF®-Certified Reader **. HTRF-specific. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. * For HTRF microplate recommendations, please visit Table 1 Instrument settings used for HTRF with terbium donor and red acceptor. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). culture plate. This protocol enables the cells' viability and confluence. Revvity. Figure 1: Principle of HTRF Cortisol competitive assay. Customer Transformation Hub. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. It is typically used to determine the concentration of nucleic acids and proteins. This protocol gives the cells viability and confluence to be monitored. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. FOR HTRF CERTIFIED READER. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. Remove the plate seal and read on an HTRF compatible reader. It continues to deliver optimal results. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. FOR HTRF CERTIFIED READER. This protocol enables the cells' viability and confluence to be monitored. Conclusion. Optimized instrument settings for the HTRF camps HiRange assay. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. In particular, these parameters should be entered as defined in the table below Setup Top filter cube EX 330 / LUM (block #18) EM 620 / 665 / LUM (block #41) Light source LaserDetect flash, glow, Dual Luciferase ® Reporter and BRET assays. Plate 25 μL of cells in. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. FOR HTRF CERTIFIED READER. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. According to the manufacturers protocol, this protein assay is linear in the range of 0. For more information. Homogeneous Time-Resolved Fluorescence (HTRF) . For more. At that stage, the analyte is sandwiched between the 2 antibodies. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. FOR HTRF CERTIFIED READER For. 9. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. CulturPlate-96: White Opaque 96-well Microplate, Sterile and Tissue Culture Treated. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. Dedicated UV-laser excitation. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. Discover our high performance reader. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Read other about this measurement approach here. Incubate 4h at RT. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. The HTRF ratio obtained represents the total binding. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. These are the different kinetic modes on BMG LABTECH microplate readers. FOR HTRF CERTIFIED READER. Incubate overnight at RT. FOR HTRF CERTIFIED. The CLARIOstar® Plus is our most flexible multi-mode plate reader. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. • HTRF®-Certified Reader. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Phospho-Rb (Ser780) two-plate assay protocol. Use simpler, faster protocols. FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Incubate overnight at RT. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. When MikroWin opens, if you already have a pre-existing template for. HTRF Setup. Assay detection requires a microplate reader with. FOR HTRF CERTIFIED READER. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. FOR HTRF CERTIFIED READER. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. CLARIOstar is equipped with a specific optical device which enables the measurement of. → Incubate 2h (if the detection is not sensitive enough, it is better to incubate 20h). Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . Microplate readers are only certified if they. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. No washing steps are required. Read & analyse. It accepts all types of microtitration plate. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. FOR HTRF CERTIFIED. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Make sure the setup for Tb Cryptate is used. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Revvity. Even if not listed, some readers may be compatible with HTRF technology. The CulturPlate is PerkinElmer’s microplate designed for cellular assays, tissue-culture treated and sterilized by gamma-irradiation to facilitate adherent cell growth. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. February 23, 2010, WINOOSKI VT, USA - The Synergy TM H4 Hybrid Microplate Reader from BioTek Instruments is now HTRF® certified. MUTANT HTT STANDARD WORKING SOLUTIONSLysis buffer 4X for two-plate assay protocol on suspension cells Determine the amount of lysis buffer needed for the experiment. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes. It can also be further streamlinedFOR HTRF CERTIFIED READER. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. The kit includes everything you need: reagents, buffers and a microplate. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. View Brochure. Introduction. An. Configure your microplate reader. Not for use in diagnostic procedures. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Make sure the setup for Tb Cryptate is used. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. Read & analyse. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. For more information about HTRF. Make sure the setup for Eu Cryptate is used. Results for HTRF Reader Control Kit with black and white microplates. 5 µL Prostaglandin E2 . For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Other technologies such as imaging. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. Incubate overnight at RT. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. • Select Excitation Filter 320x40 HTRF Eu cryptate* • Select Excitation Optic Empty • Select Emission Filter 495xm10uv • Select Emission Optic 2 – FPThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. After an overnight incubation, the plate is washed 3 times. FOR HTRF CERTIFIED READER. This work reports 25 small-molecule binders of the m 6 A-reader domain of YTHDC1. Quantify up to 10 analytes per well. Preconfigured HTRF protocols in SoftMax ® Pro Software 7. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. MATERIALS PROVIDED: KIT . This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. $352. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. * For HTRF microplate recommendations, please visit . The donor and acceptor can be used to label a variety of biomolecules, for applications. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. no. Lysates are then transferred to the assay plate for the detection of total-Tau by HTRF. FOR HTRF CERTIFIED READER For more information about HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Cover the plate with a plate sealer. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For more information about HTRF. Incubate 4h at RT. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. Incubate 4h at RT. Make sure the setup for Tb Cryptate is used. The detection antibody is incubated for 1. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. pre+ 5 µL HTRFcompounds Activation Buffer. Plate 25 μL of cells in. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. HTRF Microplate Readers. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. Bench top solutions for cell based assays. HTRF-specific. For more information about HTRF. + 4 µL HTRF ® pre-mixed antibodies . Excitation lasers significantly improve performance and lower. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit . + 4 µL HTRF pre-mixed antibodies . EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. PerkinElmer. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. 0. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. The optimized well design and high quality plastics provides low background and crosstalk. Remove the plate seal and read on an HTRF compatible reader. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. If not, our scientific team will help you choose the instrument that best suits your needs and budget. TO PREPARE TISSUE SAMPLES: Please refer to the Technical Note “Optimize Your HTRF® Cell Signaling Assays on Tissues” onHow to use Synergy microplate readers for HTRF detection, a versatile technology for drug discovery and life science research. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Read & analyse. Certified for use with Alpha, LANCE ® or DELFIA ®. Plate 25 μL of cells in. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Assay plates were incubated at RT and read at different time points thereafter on an HTRF ® certified. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. These plates can be read on all HTRF certified readers, thus making their implementation in. Remove the plate sealer and read on an HTRF® compatible reader . How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 1. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. + 4 µL HTRF ® pre-mixed antibodies . FOR HTRF CERTIFIED. The adapterResults summary for HTRF IP1 standard curve. Read & analyse. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Read & analyse.